Summary
Overview
Work history
Education
Skills
Accomplishments
Publications
Certification
Additional roles at work
Affiliations
Languages
Timeline
Generic
Anwesha Sarkar

Anwesha Sarkar

Warsaw

Summary

Highly skilled in molecular biology and cell culture techniques, with extensive expertise in Caenorhabditis elegans research, protein analysis, and genetic manipulation. Proficient in advanced methodologies such as confocal microscopy, RNA extraction, immunohistochemistry, and flow cytometry using BD FACS Aria. Demonstrates strong capabilities in DNA cloning, mutagenesis, RNAi gene depletion, and immunoprecipitation to drive innovative scientific investigations. Adept at processing primary tissue and conducting protein estimation using BCA assay to support high-impact research objectives. Committed to advancing scientific knowledge through meticulous experimentation and collaborative efforts within the field of molecular biology.

Overview

11
11
years of professional experience
5
5
years of post-secondary education
1
1
Certification

Work history

PhD student

International Institute of Molecular and Cell Biology
Warsaw
03.2021 - 03.2025

Research Intern

International Institute of Molecular and Cell Biology
Warsaw
02.2020 - 02.2021

Research Intern

University of Warsaw
Warsaw
10.2019 - 01.2020

Project Research Fellow

National Institute of Biomedical Genomics
, India
05.2015 - 12.2017

Teaching Assistant

Presidency University
, India
07.2014 - 04.2015

Education

Master of Science - Molecular Biology and Genetics

Presidency University
06.2012 - 06.2014

Bachelor of Science with Honours - Biochemistry

University of Calcutta
07.2009 - 06.2012

Skills

  • Caenorhabditis elegans
  • Proteins
  • Cell culture
  • Microscopy
  • DNA/RNA
  • Synchronization and manipulations in solid and liquid cultures
  • Protein estimation using BCA assay
  • Processing of primary tissue
  • Immunohistochemistry
  • RNA extraction
  • Confocal microscopy
  • SDS-PAGE
  • Western Blot
  • Flow Cytometry using BD FACS Aria
  • Phase-contrast microscopy
  • DNA cloning/mutagenesis
  • SUnSET assay
  • Immuno-precipitation
  • Transfection
  • PCR / qPCR
  • RNAi gene depletion
  • Immunofluorescence
  • Immunoprecipitation
  • Genetic crosses in C elegans

Accomplishments

1. First prize in Best Poster Competition at IIMCB Retreat in September 2024.

2. First prize for best talk and oral presentation at PhD students’ Report Session, IIMCB, June 2022.

3. Third prize for best talk and oral presentation at PhD students’ Report Session, IIMCB, June 2021.

INSPIRE Fellowship (Provisional offer) from Department of Science and Technology (DST-INSPIRE), Government of India, August 2016.

Gold medal for 1st rank in Master of Science (MSc), Presidency University from the Governor of West

Bengal, India. December 2014.

4. Prof. Shivatosh Mookerjee Memorial Book Prize for 1st rank in MSc, Molecular Biology and Genetics from Presidency University, West Bengal, India. December 2014.

Publications

1. Kutzner CE, Bauer KC, Lackmann JW, Acton RJ, Sarkar A, Pokrzywa W & Hoppe T. 2024. Optogenetic induction of mechanical muscle stress identifies myosin regulatory ubiquitin ligase NHL-1 in C. elegans. Nat Commun 15, 6879

2. Dubey AA, Sarkar A, et al., 2024.Floxuridine supports UPS independent of germline signaling and proteostasis regulators via involvement of detoxification in C. elegans. Plos Genetics Jul 31;20(7):e1011371

3. Kavya Vipparthi, Ankit Kumar Patel, Subhashis Ghosh, Subrata Das, Chitrarpita Das, Koyeli Das, Anwesha Sarkar, Venu Thattikonda, Biswajoy Pal, Arun Sasikumaran Nair Remani, Neeraj Arora, Mayur Parihar, Maleppillil Vavachan Vijayakumar, Manoj Kumar Bhat, Ramanamurthy Boppana, Samsiddhi Bhattacharjee, Nidhan Kumar Biswas, Pattathyil Arun, Rajeev Sharan, Sandeep Singh. (2020). Two novel cell culture models of buccal mucosal oral cancer from patients with no risk habits of tobacco smoking or chewing.

Certification

1. Research meet on Exploring Proteostasis and Mechanical Stress Management: Lessons from Caenorhabditis elegans at CECAD, Cologne, Germany, August 2023

2. Greig-NCN grant mini symposium at University of Oslo, Norway, March 2024

3. EMBO Ribosome Profiling Workshop, April 2022.

Additional roles at work

· Lab Representative for PhD students at the Laboratory of Protein Metabolism, IIMCB from 2021-2024

· Co-ordinator and Participant in Cultural Program for Institute Day at National Institute of Biomedical Genomics (NIBMG), India.

· Student Representative from Graduate studies for DBT-builder meet for sanction of project funds at Presidency University, West Bengal, India.

· Head Girl of Graduate Studies, Class of 2014, Presidency University, West Bengal, India.

· Head Girl of Undergraduate Studies, Class of 2012, Asutosh College under Calcutta University.

Affiliations

  • Cooking, Gardening, Travelling, Cultural activities

Languages

English
Fluent

Timeline

PhD student

International Institute of Molecular and Cell Biology
03.2021 - 03.2025

Research Intern

International Institute of Molecular and Cell Biology
02.2020 - 02.2021

Research Intern

University of Warsaw
10.2019 - 01.2020

Project Research Fellow

National Institute of Biomedical Genomics
05.2015 - 12.2017

Teaching Assistant

Presidency University
07.2014 - 04.2015

Master of Science - Molecular Biology and Genetics

Presidency University
06.2012 - 06.2014

Bachelor of Science with Honours - Biochemistry

University of Calcutta
07.2009 - 06.2012
Anwesha Sarkar