Lakshmipathy Muthukrishnan
Poznan
Summary
Hardworking Postdoctoral Research Fellow with demonstrated record of research success in Microbiology, Microbial and Plant Nanotechnology with 16 years of experience. Self-directed and motivated professional with highly-ethical, meticulous and thorough approach.
Overview
16
16
years of professional experience
Work History
Post-Doctoral Researcher
IPG-PAS
08.2023 - Current
- Perform micropropagation and maintenance of Hypericum perforatum in MS culture medium
- Perform agrobacterium mediated transformation by co-culture technique to revoke recalcitrance in Hypericum plants
- Develop protocols based on green chemistry principles to synthesize metal nanoparticles such as silver, gold, zinc and copper using secondary metabolites extracted from Hypericum perforatum
- Characterization and interpretation of data from spectroscopic and electron microscopic analyses
- Devise strategies to extract cellulose and lignin from Hypericum plant for sustainable agriculture applications
- Develop bionanocomposite based approach in formulating nanofertilizers in a circular fashion
- Release profile analysis of Zn / Cu / Fe / Ag / Au ions from bionanocomposite using T-XRF (S2 picofox) spectrometer
- Drafting manuscripts, conference presentations and guiding students’ projects
Assistant Professor (Research)
Saveetha University
06.2020 - 06.2023
- Company Overview: Chennai, India
- Project Lead as Principal Investigator in a grant approved by SERB, Government of India worth INR 2 300 000
- Devising a nanotechnology based strategy to encounter biofilm forming dental pathogens such as Streptococcus pyogenes and Enterococcus faecalis
- Study design and protocol optimization for biofilm inhibition using green synthesized nanoparticles as intracanal medicament in treating endodontic infections
- Proficient in drafting research proposals, manuscripts and book chapters to disseminate knowledge among scientific fraternity
- Designing a study for Ph.D scholars, troubleshooting the protocol, reviewing the progress and communicating the work to the journal
- Ph.D co-ordinator in-charge of assessing the progress by conducting review meetings, review board members panel selection and supporting the research department
- Chennai, India
CSIR - Research Associate
CSIR-CLRI
04.2017 - 04.2020
- Company Overview: Chennai, India
- Study design on fabrication of nanocomposite to extend the shelf life of skins for quality leather production
- Successfully optimized a protocol for the short term preservation of skins using Ag@ZnO nanocomposite as an alternative to conventional salting process
- Assisting scientist in drafting a proposal, supervising research scholars, troubleshooting the experiments, manuscript submission and publication
- Develop skill sets by collaborating with the laboratory of other research institutions as needed
- Chennai, India
Research Associate - ICMR
King Institute of Preventive Medicine & Research
07.2016 - 03.2017
- Company Overview: India
- Propagation of influenza A virus in MDCK and / or Vero cell line
- Performing diagnostic tests for Hepatitis, Parvo, Dengue viruses using ELISA and PCR
- Validating the kit based on the sensitivity to viruses
- Perform RT-PCR based detection of influenza A virus
- India
Research Associate - DBT
Sathyabama University
07.2015 - 06.2016
- Company Overview: India
- Assisting the PI in fabricating metal nanoparticles to encounter multidrug resistant pathogens such as Methicillin resistant Staphylococcus aureus (MRSA) and Klebsiella pneumoniae
- Understanding the mechanism of action by analytical and molecular based approach
- Mentoring the students’ dissertation and providing hands-on training toward nanoparticle synthesis and characterization
- India
Teaching Research Assistant (TRA)
Sathyabama University
12.2010 - 06.2015
- Company Overview: India
- Worked in DBT project on the fabrication of silver nanoparticles and assessing the level of toxicity for use as an antibacterial material against antibiotic resistant bacterial strains (MRSA and Klebsiella pneumoniae)
- Nanoparticle synthesis using bacterial enzyme and characterization studies using spectroscopic and electron microscopic analyses
- Genotoxic study of nanoparticles by agarose gel electrophoresis
- In vitro compatibility study of nanoparticles using MTT assay and fluorescent based assay for deciphering the apoptotic mechanism
- Pursued Ph.D and independent research by designing the study and standardizing the protocols
- As an academician, handled Environmental science and engineering module for B.E / B.Tech undergrad students
- Drafted external projects such as Indo-Korean and Indo-Russian with other research institutes as needed
- India
Research Assistant
King Institute of Preventive Medicine & Research
08.2008 - 11.2010
- Company Overview: India
- Associated in World Health Organization (WHO) funded National Polio Surveillance Project aimed at monitoring the circulation of virulent strains of polio virus in different geographical locations
- Followed GLPs guidelines framed by the WHO for working in BSL2 laboratory
- Proficient in handling and seeding L20B and RD cell lines for the propagation of Polio virus
- Processing of samples, inoculation of the virus into the cell line and observation of cytopathic effect induced by virus
- Experience in media preparation, seeding, maintenance and cryopreservation of various cell lines such as Hep2, HepG2, A549, C636, Vero, VeroSLAM, MDCK etc
- Performed neutralization and haemagglutination test for viruses
- Conducted in vitro cytotoxicity study (MTT assay) and fluorescent staining based techniques
- Performed ELISA and PCR for the diagnosis of Enteroviruses, parvoviruses and influenza A virus
- Provide regular monitoring and management of equipments used in the department
- India
Education
Ph.D - Life Sciences
Sathyabama University
09.2016
M.Sc. - Applied Microbiology
Jaya College of Arts And Science
04.2008
B.Sc. - Botany
Pachaiyappa’s College
10.2004
Skills
- Scientific Writing
- Laboratory techniques
- Research
- Proficient in Microbiology, Microbial and Plant Nanotechnology
- Interpersonal and written communication
- Interpersonal Skills
Accomplishments
- Independent researcher received a start-up research grant worth INR 2,300,000 from DST-SERB, Govt. of India.
Presidentscout
Rastrapathi Awardee
Patents
- Nanozyme based Antibacterial for Countering Biofilm, Lakshmipathy Muthukrishnan and Deepak Nallasamy, 202241026099, 2022
- Biodegradable Nanopolymer Encapsulated Periostracum Based Antibiofilm Solution, Annie Sylvia, Lakshmipathy Muthukrishnan, Krithika Datta, Mukesh Doble and Deepak Nallasamy, 202241056228, 2022
Publications
30+ Publications in Peer reviewed journals, 1 Research Grant, 2 Patents, 7 Book chapters, https://orcid.org/0000-0001-7065-1608
Languages
Telugu
Native language
Hindi
Intermediate
B1
French
Elementary
A2
English
Proficient
C2
Timeline
Post-Doctoral Researcher
IPG-PAS
08.2023 - Current
Assistant Professor (Research)
Saveetha University
06.2020 - 06.2023
CSIR - Research Associate
CSIR-CLRI
04.2017 - 04.2020
Research Associate - ICMR
King Institute of Preventive Medicine & Research
07.2016 - 03.2017
Research Associate - DBT
Sathyabama University
07.2015 - 06.2016
Teaching Research Assistant (TRA)
Sathyabama University
12.2010 - 06.2015
Research Assistant
King Institute of Preventive Medicine & Research
08.2008 - 11.2010
Ph.D - Life Sciences
Sathyabama University
M.Sc. - Applied Microbiology
Jaya College of Arts And Science
B.Sc. - Botany
Pachaiyappa’s College
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