Sakshi Sareen
Scientist
Warsaw
Summary
Second year Ph.D. student at Institute of physical chemistry, working on assessing changes in biophysical properties during cell death in cancer (two publications underway).
Overview
6
6
years of professional experience
4
4
years of post-secondary education
Work History
Senior Research Associate
Syngene International
Bengaluru
06.2019 - 12.2020
- Tested bispecific ligand to increase cytotoxicity against tumor cells using CAR-T technology
- Animal Handling: Immunization of Balb/c and C57 mice with different antigens using RIMMS protocol
- Hybridoma technique: Performed fusion of splenocytes with SP2AB myeloma partner to develop monoclones expressing the desired antibody
- Single cell sorting and culturing of primary T cells
- FACS and flow cytometry screening of monoclones and single cells
- ELISA for assessing antibody titers
- Client interaction for data presentation
Research Trainee
Dabur India
11.2018 - 05.2019
- Developed novel formulations in personal care cosmetics and OTC drugs like Ketoconazole
- Researched the morphology of skin and hair by experimental analyses
- Strengthening and structuring product claims to increase market value.
University of Hull
09.2016 - 09.2017
- Studied cytotoxic ability of NK cells on K562 cells using FACS in cancer patients
- CFSE and Live/dead cell (fluorescent) staining and analysis (FACS)
- Standardized the protocol of NK cell isolation from PBMCs using magnetic separation method
- Individually presented a paper on Image guided surgery, highlighting the applications of Laparoscopic lymph node dissection to a panel of 20.
University of Delhi
06.2011 - 06.2014
- Researched effects of efflux pumps in Mycobacterium on ejection of antibiotics like fluoroquinolones using bioinformatics
- Protein extraction, quantification, and isolation using spectrophotometer and ELISA
- Learnt Karyotyping, pedigree analysis, plasmid, and DNA/RNA isolation techniques using gel electrophoresis.
Education
Master of Science - Biomedical Science
University of Hull
United Kingdom 04.2016 - 04.2017
Bachelor of Science - Biomedical science
University of Delhi
India 04.2011 - 04.2014
Skills
- Communication and interactions
Accomplishments
Warsaw for Ph.D. Scholar
Institute of Physical chemistry, Warsaw
Opus National Grant Fellow, National science center
Soft matter group, ICHF
Commonwealth shared Scholar, United Kingdom
University of Hull, Biomedical Science group
Women in science, Chair of Networking committee
Warsaw, Poland
British Red Cross Volunteer/Women’s group Leader
United Kingdom
Elite Fellow, DST, India
University of Delhi
Timeline
Senior Research Associate
Syngene International
06.2019 - 12.2020
Research Trainee
Dabur India
11.2018 - 05.2019
University of Hull
09.2016 - 09.2017
Master of Science - Biomedical Science
University of Hull
04.2016 - 04.2017
University of Delhi
06.2011 - 06.2014
Bachelor of Science - Biomedical science
University of Delhi
04.2011 - 04.2014
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